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microbio spin gel filtration columns  (Bio-Rad)


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    Structured Review

    Bio-Rad microbio spin gel filtration columns
    Microbio Spin Gel Filtration Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microbio+spin+gel+filtration+columns/Micro+Bio-Spin+P-30+Gel+Columns/pm35871304-52-9-14
    Average 96 stars, based on 364 article reviews
    microbio spin gel filtration columns - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Labeling:

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. 5ʹ biotinylated and 3ʹ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for four hours at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange into ddH 2 O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
    Article Snippet: .. 5′ biotinylated and 3′ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for 4 h at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange D ow nloaded from https://academ ic.oup.com /nar/article/50/14/8240/6649371 by guest on 02 Septem ber 2024 into ddH2O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. 5′ biotinylated and 3′ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for 4 h at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange into ddH 2 O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Buffer Exchange:

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. 5ʹ biotinylated and 3ʹ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for four hours at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange into ddH 2 O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
    Article Snippet: .. 5′ biotinylated and 3′ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for 4 h at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange D ow nloaded from https://academ ic.oup.com /nar/article/50/14/8240/6649371 by guest on 02 Septem ber 2024 into ddH2O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. 5′ biotinylated and 3′ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for 4 h at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange into ddH 2 O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Filtration:

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. 5ʹ biotinylated and 3ʹ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for four hours at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange into ddH 2 O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
    Article Snippet: .. 5′ biotinylated and 3′ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for 4 h at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange D ow nloaded from https://academ ic.oup.com /nar/article/50/14/8240/6649371 by guest on 02 Septem ber 2024 into ddH2O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
    Article Snippet: .. Unreacted nucleotides were removed from the transcription mixture with MicroBio-Spin gel filtration columns (P30, Bio-Rad), and the RNA product was then precipitated using 12 volumes of 7.5 M lithium chloride. ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. Unreacted nucleotides were removed from the transcription mixture with MicroBio-Spin gel filtration columns (P30, Bio-Rad), and the RNA product was then precipitated using 1⁄2 volumes of 7.5M lithium chloride. ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. Unreacted nucleotides were removed from the transcription mixture with MicroBio-Spin gel filtration columns (P30, Bio-Rad), and the RNA product was then precipitated using \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$\frac{1}{2}$\end{document} volumes of 7.5 M lithium chloride. ..

    Article Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation
    Article Snippet: .. 5′ biotinylated and 3′ amino-modified oligonucleotides purchased from IDT were reacted with a 1:8 molar ratio of oligonucleotide to NHS-ester derivatives of Cy5 (for intramolecular FRET experiments with dual labeled RNA), Cy3 and Cy3.5 in 0.1 M sodium bicarbonate for 4 h at room temperature, followed by four successive chloroform extractions to remove unreacted dye, and by buffer-exchange into ddH 2 O using MicroBio-Spin gel filtration columns (P30, Bio-Rad). ..



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